I kappa B Kinase Detection Set

• Background:

  For images please see PDF data sheet

• Reactivity:

  Human

• Immunogen:

  Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

• Clonality:

  Polyclonal

• Type:

  Detection Sets

• Field of Research:

  Inflammasomes

• Purification:

  Antibodies are supplied as affinity chromatography purified IgG.

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  PBS containing 0.02% sodium azide.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable at 4˚ C for three months, store at -20˚ C for up to one year.

• Applications Notes:

  These polyclonal antibodies can be used for detection of IKKα , IKKβ , IKKγ and IKKε by immunoblot at 0.5 - 2 μ g/mL, and by immuncytochemistry (IKKα , IKKβ and IKKγ ) or immunohistochemistry (IKKε ) at 1 - 10 μ g/mL, and Immunoflourescence.

• Background Reference 01:

  DiDonato JA, Hayakawa M, Rothwarf DM, et al. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997; 388:548-54.

• Background Reference 02:

  Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IkappaB kinase. Cell 1997; 90:373-83.

• Background Reference 03:

  Zandi E, Rothwarf DM, Delhase M, et al. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997; 91:243-52.

• Background Reference 04:

  Woronicz JD, Gao X, Cao Z, et al. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997; 278:866-9.

• Tested Applications:

  WB, IHC, ICC, IF

• Physical Properties:

  Liquid