B7-H4 Recombinant Protein

• Background:

  V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs) . VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

• NCBI Gene ID:

  79679

• Swiss Prot:

  Q7Z7D3

• Accession Number:

  NP_078902.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Innate Immunity

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  51.8 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.8 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  V-set domain containing T cell activation inhibitor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Salceda S., et al., 2005, Exp. Cell Res. 306:128-141.

• Background Reference 02:

  Kryczek I., et al., 2006, J. Exp. Med. 203:871-881.

• Background Reference 03:

  Miyatake T., et al., 2007, Gynecol. Oncol. 106:119-127.

• Other Product Names:

  B7-H4, VTCN1, B7S1, B7h.5

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3