LIGHT / TNFSF14 Recombinant Protein

• Background:

  Tumor necrosis factor ligand superfamily member 14 (LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR) . LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.

• NCBI Gene ID:

  8740

• Swiss Prot:

  O43557

• Accession Number:

  NP 003798.2

• Tag:

  Mouse IgG Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  45.1 kDa

• Applications Notes:

  This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  TNF superfamily member 14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mauri DN., et al. 1998, Immunity., 8, 21-30.

• Background Reference 02:

  Granger SW., et al. 2003, Cytokine Growth Factor Rev., 14 (3-4), 289-96.

• Background Reference 03:

  Pasero C., et al. 2009, Curr Mol Med., 9 (7), 911-27.

• Other Product Names:

  CD258 antigen, CD258, HVEM-L, LIGHT, LTg, TNFSF14, TR2, HVEML

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3