TFPI Recombinant Protein

• Background:

  Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF) ; The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

• NCBI Gene ID:

  7035

• Swiss Prot:

  P10646

• Accession Number:

  NP_006278.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Homeostasis, Signal Transduction

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  30 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bajaj MS., et al., 2001, Thromb Haemost., 86:959.

• Background Reference 02:

  Mine S., et al., 2002, Biochemistry, 41:78.

• Background Reference 03:

  Witt I., et al., 2002, Hamostaseologie, 22:30–35.

• Other Product Names:

  TFPI, LACI, TFPI1, EPI, TFI

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3