CD3 epsilon&CD3 gamma Heterodimer Recombinant Protein

• Background:

  T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

• NCBI Gene ID:

  916

• Swiss Prot:

  P07766

• Accession Number:

  NP_000724.1 (CD3E) & AAI13831.1 (CD3G)

• Tag:

  His Tag & Fc Tag & Flag Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  39.2 kDa (CD3E) & 38.6 kDa (CD3G)

• Applications Notes:

  Human CD3E&CD3G Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G) . Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhist

• NCBI Official Name:

  CD3e molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gold DP, et al., 1986, Nature 321 (6068) : 431–4.

• Background Reference 02:

  Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21) : 8156–60.

• Background Reference 03:

  Gil J, et al., 2011, J Clin Invest, 121 (10) :3872-6.

• Background Reference 04:

  Lu X, et al.,2011, Hematology, 16 (3) :185-9.

• Other Product Names:

  CD3 epsilon & CD3 gamma, CD3E & CD3G

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3