CD3 Epsilon Recombinant Protein

• Background:

  CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

• NCBI Gene ID:

  916

• Swiss Prot:

  P07766

• Accession Number:

  NP_000724.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Immunology, Neuroscience, Signal Transduction, Stem Cell, Other

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  12.6 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  CD3e molecule, epsilon (CD3-TCR complex)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gold DP, et al., 1986, Nature 321 (6068) : 431–4.

• Background Reference 02:

  Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21) : 8156–60.

• Background Reference 03:

  De Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10) : 1512–7.

• Background Reference 04:

  Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1) : 69–73.

• Other Product Names:

  FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3