S100B Recombinant Protein

• Background:

  S-100 protein beta chain (S100B), a member of the S-100 family, is also known as S-100 protein subunit beta and S100 calcium-binding protein B, which contains two EF-hand domains. S100B can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Also, S100B can bind to and initiate the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction, S100B may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Fuethermore, S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

• NCBI Gene ID:

  6285

• Swiss Prot:

  P04271

• Accession Number:

  NP_006263

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Cell Cycle, Neuroscience, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  11.5 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  S100 calcium binding protein B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yamaguchi F., et al., 2012, J. Biol. Chem. 287:13787-13798.

• Background Reference 02:

  Smith S.P., et al., 1998, Structure 6:211-222.

• Background Reference 03:

  McClintock K.A., et al., 2003, J. Biol. Chem. 278:6251-6257.

• Other Product Names:

  S100B, NEF, S100, S100beta

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3