DKK 3 Recombinant Protein

• Background:

  Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

• NCBI Gene ID:

  27122

• Swiss Prot:

  Q9UBP4

• Accession Number:

  NP_001018067.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  37.6 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Dickkopf WNT signaling pathway inhibitor 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Medinger M, Tzankov A, et al., 2011, Thromb Haemost. 105:72-80.

• Background Reference 02:

  Yue W, Sun Q, et al., 2008, Carcinogenesis. 29 (1) :84-92..

• Background Reference 03:

  Pinho S, Niehrs C.et al., 2007, Differentiation. 75 (10) :957-67.

• Other Product Names:

  DKK3, REIC, RIG

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3