PH20 Hyaluronidase Recombinant Protein

• Background:

  Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4) -linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

• NCBI Gene ID:

  6677

• Swiss Prot:

  P38567

• Accession Number:

  AAH26163

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 100 mM NaCl, pH7.0-7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  52.1 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lin Y., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075.

• Background Reference 02:

  Gmachl M., et al., 1993, FEBS Lett. 336:545-548.

• Background Reference 03:

  Arming S., et al., 1997, Eur. J. Biochem. 247:810-814.

• Other Product Names:

  SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3