CD14 Recombinant Protein

• Background:

  Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14) . Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa) . CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

• NCBI Gene ID:

  929

• Swiss Prot:

  P08571

• Accession Number:

  AAH10507

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Apoptosis, Immunology, Innate Immunity, Signal Transduction

• Purity:

  >98% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  36.6 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  CD14 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kirkland TN, Viriyakosol S, et al., 1998, Prog. Clin. Biol. Res. 397: 79–87.

• Background Reference 02:

  Kitchens RL., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 61–82.

• Background Reference 03:

  Tapping RI, Tobias PS., 2000, Chem. Immunol.. Chemical Immunology and Allergy 74: 108–21.

• Background Reference 04:

  Thomas, Celestine J., et al., 2002, FEBS Lett. (Netherlands) 531 (2) : 184–8.

• Other Product Names:

  CD14

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3