CD4 Fragment Recombinant Protein

• Background:

  CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response (1) . On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120 (2) . This soluble CD4 fragment (sCD4) will work in HIV neutralization assays at levels comparable to previously published results. The minor upper band seen in the Coomassie blue-stained gel is likely to be due to the presence of uncleaved signal sequence.

• NCBI Gene ID:

  920

• Swiss Prot:

  P01730

• Accession Number:

  NP_000607

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Immunology

• Purity:

  ~90%

• Buffer:

  100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  27 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  CD4 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bowers K, Pitcher C, and Marsh M. CD4 : a co-receptor in the immune response and HIV infection. Int. J. Biochem. Cell Biol. 1997 ; 29 :871-5.

• Background Reference 02:

  Arthos J, Deen KC, Chaikin MA, et al. Identification of the residues in human CD4 critical for the binding of HIV. Cell 1989; 57:469-81.

• Other Product Names:

  CD4 Antibody : CD4mut

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Protein ID:

  10835167

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3