EndoG Fragment Recombinant Protein

• Background:

  The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3) . EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2) . EndoG also cleaves DNA in vitro (4) .

• NCBI Gene ID:

  2021

• Swiss Prot:

  Q14249

• Accession Number:

  Q14249

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Apoptosis, Cancer

• Purity:

  ~95%

• Buffer:

  3

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  15 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Endonuclease G

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.

• Background Reference 02:

  Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans . Nature 2001; 412:90-4

• Background Reference 03:

  Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.

• Background Reference 04:

  Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.

• Other Product Names:

  EndoG Antibody : Endo G

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Protein ID:

  24638471

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3