MyD88 Fragment Recombinant Protein

• Background:

  The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway (1) . MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

• NCBI Gene ID:

  4615

• Swiss Prot:

  Q99836

• Accession Number:

  AAC50954

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Innate Immunity, Signal Transduction, Inflammasomes

• Purity:

  ~95%

• Buffer:

  3

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  15 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Myeloid differentiation primary response gene (88)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.

• Background Reference 02:

  Adachi O, Kawai T, Takeda K, et al. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998; 9:143-50.

• Background Reference 03:

  Medzhitov R, Preston-Hurlburt P, Kopp E, et al. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways. Mol. Cell 1998; 2:253-8.

• Background Reference 04:

  Kawai T, Adachi O, Ogawa T, et al. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 1999; 11:115-22.

• Other Product Names:

  MyD88 Antibody : MYD88D

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Protein ID:

  1814020

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3