PUMA Fragment Recombinant Protein

• Background:

  Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3) . PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- (1) . PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043) .

• NCBI Gene ID:

  27113

• Swiss Prot:

  Q9BXH1

• Accession Number:

  Q9BXH1

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Apoptosis, Autophagy, Cancer, Neuroscience, Other

• Purity:

  ~95%

• Buffer:

  3

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  15 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  BCL2 binding component 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.

• Background Reference 02:

  Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.

• Background Reference 03:

  Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

• Other Product Names:

  PUMA Antibody : JFY1, PUMA, JFY-1

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Protein ID:

  56748610

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3