B7-H7 / HHLA2 Recombinant Protein

• Background:

  B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.

• NCBI Gene ID:

  11148

• Swiss Prot:

  Q9UM44

• Accession Number:

  NP_009003.1

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Purity:

  >90% as determined by reduced SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  63.4 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  HERV-H LTR-associating 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ni L., et al., 2017, Mol. Cancer. Ther. 16:1203-1211.

• Background Reference 02:

  Jung K., et al., 2013, Immune. Netw. 13:184-193.

• Background Reference 03:

  Janakiram M., et al., 2015, Clin. Cancer. Res. 21:2359-2366.

• Other Product Names:

  B7-H7, HHLA2, B7 Homolog 7

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3