Autotaxin Recombinant Protein

• Background:

  Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

• NCBI Gene ID:

  5168

• Swiss Prot:

  Q13822

• Accession Number:

  NP_001035181

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Neuroscience, Obesity, Signal Transduction, Other

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 150 mM NaCl, pH 7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  94.5 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Ectonucleotide pyrophosphatase/phosphodiesterase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Stracke M.L., et al., 1992, J. Biol. Chem. 267:2524-2529.

• Background Reference 02:

  Nam S.W., et al., 2001, Cancer Res. 61:6938-6944.

• Background Reference 03:

  Hausmann J., et al., 2011, Nat. Struct. Mol. Biol. 18:198-204.

• Other Product Names:

  ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3