Propidium monoazide

CAT: 0804-HY-D1444Size: 1 mgDry Ice: NoHazardous: No
CAT#:0804-HY-D1444Size:1 mg
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Description
Propidium monoazide is a photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR[1]. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
CAS Number
[91416-20-5]
UNSPSC
12171500
Target
Bacterial; Fluorescent Dye
Type
Dye Reagents
Related Pathways
Anti-infection; Others
Field of Research
Infection
Assay Protocol
https://www.medchemexpress.com/propidium-monoazide.html
Purity
99.30
Solubility
DMSO : 2 mg/mL (ultrasonic)
Smiles
[N-]=[N+]=NC1=CC2=C(C3=C([N+](CCC[N+](CC)(CC)C)=C2C4=CC=CC=C4)C=C(N)C=C3)C=C1.[Cl-].[Cl-]
Molecular Formula
C27H32Cl2N6
Molecular Weight
511.49
References & Citations
[1]Mohamed Askar 1, et al. Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision. Mol Biol Rep. 2019 Dec;46 (6) :6463-6470.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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