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Propidium (Iodide) (solution)

CAT: 0804-HY-DY1006-01Size: 1 mLDry Ice: NoHazardous: No
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CAT#:0804-HY-DY1006-01Size:1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Propidium Iodide (PI) (solution) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.Solvent and Concentration: Sterile water: 1 mg/mL
CAS Number
25535-16-4
Target
DNA/RNA Synthesis; Fluorescent Dye
Related Pathways
Cell Cycle/DNA Damage; Others
Field of Research
Others
Smiles
C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]
Molecular Formula
C27H34I2N4
Molecular Weight
668.41
References & Citations
[1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43 (10) :1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20°C for 1 year, protect from light. Avoid repeated freezing and thawing
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Propidium Iodide

Propidium iodide is an organic iodide salt. It contains a propidium.

CAS Number25535-16-4
PubChem CID104981
IUPAC Name3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium diiodide
Molecular FormulaC27H34I2N4
Molecular Weight668.4
Topological Polar Surface Area55.9
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count4
Rotatable Bond Count7
Heavy Atom Count33
Formal Charge0
Complexity555
SMILES
CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
InChI
InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChIKey
XJMOSONTPMZWPB-UHFFFAOYSA-M
Chemical Structure
2D Structure
2D structure of Propidium Iodide
CAS: 25535-16-4
CID: 104981
Formula: C27H34I2N4
MW: 668.4
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight668.4
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count4
Rotatable Bond Count7
Exact Mass668.08729
Monoisotopic Mass668.08729
Topological Polar Surface Area55.9 Ų
Heavy Atom Count33
Formal Charge0
Complexity555
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count3
Compound Is CanonicalizedYes

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