LARGE Antibody

• Gene Name :

  LARGE xylosyl- and glucuronyltransferase 1

• Gene Aliases :

  Acetylglucosaminyltransferase-like 1A; acetylglucosaminyltransferase-like protein; Glycosyltransferase-like protein; glycosyltransferase-like protein LARGE1; LARGE; LARGE xylosyl- and glucuronyltransferase 1; MDC1D; MDDGA6; MDDGB6.

• Gene ID :

  9215

• Accession Number :

  NP_004728.1NP_598397.1XP_005261888.1XP_005261889.1

• Reactivity :

  Human

• Immunogen :

  Recombinant fragment of human LARGE1 (amino acids 35-142)

• Target :

  Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4- (phosphate-6-) mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806) . Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699) .

• Clonality :

  Monoclonal

• Clone :

  LARGE-02

• Type :

  Monoclonal Antibody

• Applications :

  Flow cytometry|Western blot

• Purification :

  Purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Buffer :

  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

• Reconstitution :

  2°C to 8°C

• Fragment :

  IgG2b

• Specificity :

  The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase localizing mainly to the Golgi apparatus. Crossreactivity with LARGE2 was not determined.

• NCBI Gene Symbol :

  LARGE1

• Host or Source :

  Mouse

• Protein Name :

  LARGE xylosyl- and glucuronyltransferase 1

• Gene Name URL :

  LARGE1

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_004737.4NM_133642.3XM_005261831.2XM_005261832.2