Propidium (Iodide)

• Description :

  Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

• UNSPSC :

  12171500

• Hazard Statement :

  H341

• Target :

  DNA/RNA Synthesis; Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Cell Cycle/DNA Damage; Others

• Applications :

  COVID-19-immunoregulation

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/Propidium_Iodide.html

• Purity :

  99.69

• Solubility :

  DMSO : 100 mg/mL (ultrasonic) |H2O : 3.57 mg/mL (ultrasonic; warming; heat to 60°C)

• Smiles :

  C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]

• Molecular Formula :

  C27H34I2N4

• Molecular Weight :

  668.39

• Precautions :

  H341

• References & Citations :

  [1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43 (10) :1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  4°C (Powder, sealed storage, away from moisture and light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [25535-16-4]