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Propidium (Iodide)

CAT: 0804-HY-D0815-01Size: 5 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-D0815-01Size:5 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
CAS Number
25535-16-4
UNSPSC
12171500
Hazard Statement
H341
Target
DNA/RNA Synthesis; Fluorescent Dye
Type
Dye Reagents
Related Pathways
Cell Cycle/DNA Damage; Others
Applications
COVID-19-immunoregulation
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Propidium_Iodide.html
Purity
99.69
Solubility
DMSO : 100 mg/mL (ultrasonic) |H2O : 3.57 mg/mL (ultrasonic; warming; heat to 60°C)
Smiles
C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]
Molecular Formula
C27H34I2N4
Molecular Weight
668.39
Precautions
H341
References & Citations
[1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43 (10) :1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139 (2) :271-9.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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