Propidium (Iodide)

• UNSPSC Description:

  Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

• Target Antigen:

  DNA/RNA Synthesis; Fluorescent Dye

• Type:

  Dye Reagents

• Related Pathways:

  Cell Cycle/DNA Damage;Others

• Applications:

  COVID-19-immunoregulation

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/Propidium_Iodide.html

• Purity:

  99.69

• Solubility:

  DMSO : 100 mg/mL (ultrasonic)|H2O : 3.57 mg/mL (ultrasonic;warming;heat to 60°C)

• Smiles:

  C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]

• Molecular Weight:

  668.39

• References & Citations:

  [1]Hezel M, et al. Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain. Micron. 2012 Oct;43(10):1031-8.|[2]A rapid and simple method for measuring thymocyte apoptosis by propidium iodidestaining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9.|[3]Nicoletti I, et al. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 Jun 3;139(2):271-9.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  4°C (Powder, sealed storage, away from moisture and light)

• Clinical Information:

  No Development Reported

• CAS Number:

  25535-16-4