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DsRed Antibody (Biotin)

CAT: 0013-GTR18184625Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184625Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RFP antibody (Biotin)
Product Name Alternative
Chicken anti-RFP antibody biotin conjugation, biotin conjugated chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
UniProt
Q9U6Y8
Reactivity
Other
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Target
DsRed
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, IF: 1:100, WB: 1:2,000 - 1:5,000
Purity
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Tested Applications
ELISA, IF, WB
Host or Source
Gallus
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgY

UniProtKB · Q9U6Y8

Red fluorescent protein drFP583

RFP_DISSP · Discosoma sp.

View on UniProt ↗
Primary accession
Q9U6Y8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Discosoma sp. (Sea anemone)
Taxonomy ID
86600
Alternative names
DsRed
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structure

Ligand

Chromophore

Biological process

Luminescence

Molecular function

Photoprotein

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