Anti-Ms CD11b FITC

Anti-Ms CD11b FITC

• Background: CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
• Specifications: The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse, Rabbit, Human, Non-human primates
• Immunogen: B10 mouse spleen cells enriched for T cells
• Target Antigen: CD11b
• Isotype: IgG2b
• Clone: M1/70
• Applications: Flow cytometry: Recommended dilution: 1-3 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J Appl Physiol. 2003 Sep95(3):969-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/12730143?ordin,*Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J Immunol. 2006 Nov 15177(10):6974-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/17082612?ordin,*Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages. Glycobiology. 2009 Mar19(3):258-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/19029201?ordin,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Ault KA, Springer TA: Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan126(1):359-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/7451976?ordina,*Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1158(2):586-602., URL: https://www.ncbi.nlm.nih.gov/pubmed/6193226?ordina,*Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar43(3):313-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/7868861?ordina,*Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 2000 Mar 1497(6):2697-702., URL: https://www.ncbi.nlm.nih.gov/pubmed/10706628?ordin,*Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 2000 Dec20(12):2553-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/11116052?ordin,*Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15168(6):3088-98., URL: https://www.ncbi.nlm.nih.gov/pubmed/11884483?ordin,*Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug8(8):539-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/81133?ordinalp,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.