Anti-Ms CD2 FITC

• Description:

  CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

• Certification:

  RUO

• Immunogen:

  Murine thymocytes

• Target Antigen:

  CD2

• Clone:

  RM2-5

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Wallin J, Eibel H, Neubüser A, Wilting J, Koseki H, Balling R: Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation. Development. 1996 Jan;122 (1) :23-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=wallin+j+eibel+h , *Altin JG, Pagler EB, Parish CR: Evidence for cell surface association of CD2 and LFA-1 (CD11a/CD18) on T lymphocytes. Eur J Immunol. 1994 Feb;24 (2) :450-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7905418, *Sasada T, Reinherz EL: A critical role for CD2 in both thymic selection events and mature T cell function. J Immunol. 2001 Feb 15;166 (4) :2394-403., URL: https://www.ncbi.nlm.nih.gov/pubmed/11160298, *Cho HJ, Edmondson SG, Miller AD, Sellars M, Alexander ST, Somersan S, Punt JA: Cutting edge: identification of the targets of clonal deletion in an unmanipulated thymus. J Immunol. 2003 Jan 1;170 (1) :10-3., URL: https://pubmed.ncbi.nlm.nih.gov/12496375/, *Fassett MS, Jiang W, D'Alise AM, Mathis D, Benoist C: Nuclear receptor Nr4a1 modulates both regulatory T-cell (Treg) differentiation and clonal deletion. Proc Natl Acad Sci U S A. 2012 Mar 6; 109 (10) :3891-6., URL: https://pubmed.ncbi.nlm.nih.gov/22345564/, *Milstein O, Tseng SY, Starr T, Llodra J, Nans A, Liu M, Wild MK, van der Merwe PA, Stokes DL, Reisner Y, Dustin ML: Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 2008 Dec 5;283 (49) :34414-22., URL: https://pubmed.ncbi.nlm.nih.gov/18826951/, *Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4 (CD244) /CD48 in the generation of NK effector functions. Blood. 2006 Apr 15;107 (8) :3181-8., URL: https://pubmed.ncbi.nlm.nih.gov/15905190/, *Cibotti R, Bhandoola A, Guinter TI, Sharrow SO, Singer A: CD8 coreceptor extinction in signaled CD4 (+) CD8 (+) thymocytes: coordinate roles for both transcriptional and posttranscriptional regulatory mechanisms in developing thymocytes. Mol Cell Biol. 2000 Jun;20 (11) :3852-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10805728 , *Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS: Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Immunity. 2001 Mar;14 (3) :253-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/11290335

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-3 μg/ml.

• Species:

  Mouse

• CAS Number:

  9007-83-4

• Isotype:

  Rat IgG2b lambda