Anti-Ms CD11a FITC

• Description:

  CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

• Certification:

  RUO

• Reactivity:

  Mouse

• Immunogen:

  C57BL/6 mouse splenic secondary cytotoxic T lymphocytes

• Target Antigen:

  CD11a

• Clone:

  M17/4

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T-lymphocyte (CTL) -mediated killing: functional screening and topographic relationship. Cell Immunol. 1982 Oct;73 (1) :1-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/6983917?itool=, *Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol. 1991 Mar 15;146 (6) :1773-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/1672331?itool=, *Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity. J Immunol. 2006 Mar 15;176 (6) :3356-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/16517703?itool, *Smith SS, Barnum SR: Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J Leukoc Biol. 2008 Jan;83 (1) :71-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17928460?itool, *Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug-induced lupus. II. T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. J Clin Invest. 1996 Jun 15;97 (12) :2866-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/8675699?itool=, *Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J Exp Med. 2002 Dec 2;196 (11) :1515-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/12461086?itool, *Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir Res. 2005 Jul 12;6:70., URL: https://www.ncbi.nlm.nih.gov/pubmed/16011799?itool, *Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol. 2001 Sep;13 (9) :1165-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/11526097?itool, *Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood. 2001 Mar 1;97 (5) :1282-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/11222371?itool

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-2 μg/ml.

• Isotype:

  Rat IgG2a