Anti-MAP2 Purified

• Description:

  MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.

• Certification:

  RUO

• Reactivity:

  Pig, Human, Mouse

• Immunogen:

  Microtubule protein (bovine brain) enriched for kinesin

• Target Antigen:

  MAP2

• Clone:

  MT-08

• Applications:

  IP, WB, IHC-P, IHC-F, ICC, ELISA

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW: Global landscape and dynamics of Parkin and USP30-dependent ubiquitylomes in iNeurons during mitophagic signaling. Mol Cell. 2020 Mar 5;77 (5) :1124-1142., URL: https://pubmed.ncbi.nlm.nih.gov/32142685/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.

• Applications Notes:

  Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: brain. Immunohistochemistry (frozen sections) : Positive tissue: murine brain. Immunoprecipitation: Positive material: porcine brain. Western blotting: Positive control: porcine brain. ELISA: Positive control: porcine brain. Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.

• Isotype:

  Mouse IgG1