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Anti-Dendra2 purified

CAT: 0270-11-836-C100Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0270-11-836-C100Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.
Certification
RUO
Reactivity
Species independent
Immunogen
Dendra2 tag
Target Antigen
Dendra2
Clone
PAb (836)
Applications
WB, ICC, FC (QC tested)
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG: Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers. Sci Rep. 2017 Sep 7;7 (1) :10899., URL: https://pubmed.ncbi.nlm.nih.gov/28883437/, *McEwen AE, Maher MT, Mo R, Gottardi CJ: E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014 Aug 15;25 (16) :2365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/24966173
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The rabbit polyclonal antibody PAb (836) recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya) .
Applications Notes
Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.
Isotype
Rabbit polyclonal

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