Anti-Dendra2 purified

Anti-Dendra2 purified

• Background: Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.
• Specifications: The rabbit Polyclonal antibody PAb (836) recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya).
• Certification: RUO
• Host: Rabbit
• Species Reactivity: Species independent
• Immunogen: Dendra2 tag
• Target Antigen: Dendra2
• Clonality: Polyclonal
• Clone: PAb (836)
• Applications: Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 1-4 μg/mL. Extracellular and intracellular staining.
• Validated Applications: WB, ICC, FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG: Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers. Sci Rep. 2017 Sep 77(1):10899., URL: https://pubmed.ncbi.nlm.nih.gov/28883437/,*McEwen AE, Maher MT, Mo R, Gottardi CJ: E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014 Aug 1525(16):2365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/24966173,
• Storage Conditions: Store at 2-8°C. Do not freeze.