Anti-BCL6 Purified

Anti-BCL6 Purified

• Background: BCL6 is a zinc finger-containing transcription factor, which acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. On B cells, BCL6 induces proliferation, antibody class switching and affinity maturation, whereas it inhibits their differentiation into plasma cells. On T cells, BCL6 induces their differentiation into T-FH cells.
• Specifications: The mouse monoclonal antibody IG191E/A8 recognizes BCL6, a transcriptional repressor serving as a master regulator of germinal center reaction.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Mouse, Human
• Immunogen: pCDNA3 plasmid containing full-length sequence of human BCL6
• Target Antigen: BCL6
• Isotype: IgG1
• Clone: IG191E/A8
• Applications: Flow cytometry: Recommended dilution: 1-4 µg/mL. Intracellular staining.
• Validated Applications: WB, IHC-P, FC, IP
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Jain S, Stock A, Macian F, Putterman C: A Distinct T follicular helper cell subset infiltrates the brain in murine neuropsychiatric lupus. Front Immunol. 2018 Mar 139:487., URL: https://pubmed.ncbi.nlm.nih.gov/29593732/,*Hoser D, Schön C, Loddenkemper C, Lohneis P, Kühl AA, Sommermann T, Blankenstein T, Willimsky G: Oncogene-specific T cells fail to eradicate lymphoma-initiating B cells in mice. Blood. 2018 Aug 30132(9):924-934., URL: https://pubmed.ncbi.nlm.nih.gov/30002144/,*Tan HX, Esterbauer R, Vanderven HA, Juno JA, Kent SJ, Wheatley AK: Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Front Immunol. 2019 Mar 2910:611., URL: https://www.ncbi.nlm.nih.gov/pubmed/30984186,*Jiang W, Wragg KM, Tan HX, Kelly HG, Wheatley AK, Kent SJ, Juno JA: Identification of murine antigen-specific T follicular helper cells using an activation-induced marker assay. J Immunol Methods. 2019 Apr467:48-57., URL: https://pubmed.ncbi.nlm.nih.gov/30802449/,
• Storage Conditions: Store at 2-8°C. Do not freeze.