Anti-Bov Ig Light Chains Purified

• Certification:

  RUO

• Reactivity:

  Sheep, Cow

• Immunogen:

  Fraction of Bovine immunoglobulin.

• Target Antigen:

  Ig Light Chains

• Clone:

  IVA285-1

• Applications:

  WB, FC, IHC-F

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Antalikova J, Simon M, Jankovicova J, Horovska L, Dusinsky R, Hluchy S: Monoclonal antibody to the light chain of bovine immunoglobulin. Hybridoma (Larchmt) . 2006 Oct;25 (5) :309-12. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17044788?dopt=

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody IVA285-1 reacts with bovine Ig (light chains) . Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

• Applications Notes:

  Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml

• Isotype:

  Mouse IgG1