Anti-Bov CD62L Purified

Anti-Bov CD62L Purified

• Background: CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
• Specifications: The antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes it is also present on a subset of NK cells and certain hematopoietic malignant cells.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Human
• Immunogen: Bovine leukocytes.
• Target Antigen: CD62L
• Isotype: IgG1
• Clone: IVA94
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IP, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076?dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204?dopt=A,*Naessens J, Nthale JM, Muiya P: Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug52(4):347-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896224?dopt=A,*Howard CJ: Ruminant cluster CD62L. Vet Immunol Immunopathol. 1996 Aug52(4):255-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896209?dopt=A,
• Storage Conditions: Store at 2-8°C. Do not freeze.