Anti-Bov CD9 Alexa Fluor® 488

Anti-Bov CD9 Alexa Fluor® 488

• Background: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
• Specifications: The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Cow
• Immunogen: Bovine thrombocytes.
• Target Antigen: CD9
• Isotype: IgG2
• Clone: IVA50
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: Alexa Fluor® 488
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Sopp P, Howard CJ: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug52(4):271-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896215?dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996), URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204?dopt=A,*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076?dopt=A,*Tomaskova J, Dusinsky R, Horovska L, Simon M: A set of monoclonal antibodies specific for bovine cell surface molecule CD9. Folia Biol (Praha). 199945(5):225-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/10730891?dopt=,*Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug52(4):363-70. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8896226?dopt=A,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.