Anti-Bov CD41/CD61 Purified

Anti-Bov CD41/CD61 Purified

• Background: CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.
• Specifications: The antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Human
• Immunogen: Bovine trombocytes.
• Target Antigen: CD41/CD61
• Isotype: IgG2
• Clone: IVA30
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IP, ICC, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Mateo A, Pintado CO, Perez de la Lastra J, Dusinsky R, Simon M, Naessens J, Llanes D: Ruminant cluster CD41/CD61. Vet Immunol Immunopathol. 1996 Aug52(4):251-3., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896208?dopt=A,*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076?dopt=A,*Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug52(4):363-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896226?dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204?dopt=A,*Simon M, Dusinsky R, Horovska L, Bilka F, Hluchy S: Immunohistochemical reactivity of anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug52(4):377-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896228?dopt=A,
• Storage Conditions: Store at 2-8°C. Do not freeze.