Anti-CSK Purified

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Human, Mouse

Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.

CSK

CSK-04

WB, IP, ICC

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Palomero T, Couronné L, Khiabanian H, Kim MY, Ambesi-Impiombato A, Perez-Garcia A, Carpenter Z, Abate F, Allegretta M, Haydu JE, Jiang X, Lossos IS, Nicolas C, Balbin M, Bastard C, Bhagat G, Piris MA, Campo E, Bernard OA, Rabadan R, Ferrando AA: Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nat Genet. 2014 Feb;46 (2) :166-70. , URL: https://www.ncbi.nlm.nih.gov/pubmed/24413734

*Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J Biol Chem. 2008 Apr 18;283 (16) :10339-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/18268019?ordin, *Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784 (1) :56-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/17905674?ordin, *Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274 (4) :2291-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9890993?ordina, *Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15 (2) :711-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/7529872?ordina, *Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365 (6442) :156-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/8371758?ordina, *Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12 (10) :4706-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/1383688?ordina

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line. Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions. Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.