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Anti-CDK1 Purified

CAT: 0270-11-115-C100Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0270-11-115-C100Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
Certification
RUO
Negative Species
Mouse, Drosophila, Rat, Xenopus
Reactivity
Cow, Non-human primates, Human
Immunogen
Bacterially expressed full-length human Cdk1 protein
Target Antigen
CDK1
Clone
POH-1
Applications
IP, WB, IHC-P, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371 , *Lukas J, Draetta G, Bartek J: Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992 Jul 1;207 (1) :169-76., URL: https://pubmed.ncbi.nlm.nih.gov/1628647/
Other References
*Liu ZG, Zhao LN, Liu YW, Li TT, Fan DM, Chen JJ: Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol. 2007 Nov 23;374 (2) :334-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/17936297?ordin, *Lindqvist A, van Zon W, Karlsson Rosenthal C, Wolthuis RM: Cyclin B1-Cdk1 activation continues after entrosome separation to control mitotic progression. PLoS Biol. 2007 May;5 (5) :e123., URL: https://www.ncbi.nlm.nih.gov/pubmed/17472438?ordin, *Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S: An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178 (2) :257-68., URL: https://www.ncbi.nlm.nih.gov/pubmed/17635936?ordin
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody POH-1 specifically detects Cdk1 (p34Cdc2; an intracellular antigen) . Staining of Cdk1 reflects the proliferating potential of respective tissue.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: thymus.Western blotting: Recommended dilution: 1-2 μg/ml.
Isotype
Mouse IgG2a

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