Anti-CDK1 Alexa Fluor® 488

Anti-CDK1 Alexa Fluor® 488

• Background: Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
• Specifications: The antibody POH-1 specifically detects Cdk1 (p34Cdc2 an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Non-human primates, Human
• Negative Species: Mouse, Drosophila, Rat, Xenopus
• Immunogen: Bacterially expressed full-length human Cdk1 protein
• Target Antigen: CDK1
• Isotype: IgG2a
• Clone: POH-1
• Applications: Immunocytochemistry: Recommended dilution 2 μg/mL intracellular staining
• Validated Applications: ICC, IHC-P
• Concentration: 0.1 mg/mL
• Format: Alexa Fluor® 488
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov15(11):909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371 ,*Lukas J, Draetta G, Bartek J: Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992 Jul 1207(1):169-76., URL: https://pubmed.ncbi.nlm.nih.gov/1628647/,
• Other References: *Liu ZG, Zhao LN, Liu YW, Li TT, Fan DM, Chen JJ: Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol. 2007 Nov 23374(2):334-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/17936297?ordin,*Lindqvist A, van Zon W, Karlsson Rosenthal C, Wolthuis RM: Cyclin B1-Cdk1 activation continues after entrosome separation to control mitotic progression. PLoS Biol. 2007 May5(5):e123., URL: https://www.ncbi.nlm.nih.gov/pubmed/17472438?ordin,*Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S: An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16178(2):257-68., URL: https://www.ncbi.nlm.nih.gov/pubmed/17635936?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.