YTHDC1 antibody

• Background:

  Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A) -containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552) . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552) . Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937) . Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937) . In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937) . May also regulate alternative splice site selection (PubMed:20167602) .

• Synonyms:

  YTH domain-containing protein 1|Splicing factor YT521 (YT521-B) |YTHDC1|KIAA1966|YT521

• Gene ID:

  91746

• UniProt:

  Q96MU7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  YTH domain containing 1

• Target:

  YTHDC1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB

• Field of Research:

  Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:1000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  110 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB