YTHDC1 polyclonal antibody

• Background:

  Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  YTHDC1; YT521; YT521-B

• Swiss Prot:

  Q96MU7

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human YTHDC1 (NP_001026902.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB,1:1000 - 1:5000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  110KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  YTHDC1 polyclonal antibody detects endogenous levels of YTHDC1 protein.

• Applications Notes:

  Western blot analysis of various lysates using YTHDC1 pAb at 1:2000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4