YTHDF2 antibody

• Background:

  Specifically recognizes and binds N6-methyladenosine (m6A) -containing RNAs, and regulates mRNA stability (PubMed:24284625, PubMed:26046440, PubMed:26318451) . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661) . Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation (PubMed:24284625, PubMed:26046440) . Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (PubMed:26458103) .

• Synonyms:

  YTH domain-containing family protein 2 (DF2) |CLL-associated antigen KW-14|High-glucose-regulated protein 8|Renal carcinoma antigen NY-REN-2|YTHDF2|HGRG8

• Gene ID:

  51441

• UniProt:

  Q9Y5A9

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  YTH domain family, member 2

• Target:

  YTHDF2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IF, IHC, IP

• Field of Research:

  Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:1000-1:10000; IF: 1:10-1:100; IP: 1:200-1:1000; IHC: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  68 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IF, IHC, IP