PARP1 antibody

PARP1 antibody

• Background: PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89kD. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.
• Synonyms: Poly [ADP-ribose] polymerase 1 (PARP-1)|ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1)|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1 (ADPRT 1)|Poly[ADP-ribose] synthase 1|Protein poly-ADP-ribosyltransferase PARP1|Poly [ADP-ribose] polymerase 1, processed C-terminus Alternative names: Poly [ADP-ribose] polymerase 1, 89-kDa form|Poly [ADP-ribose] polymerase 1, processed N-terminus (NT-PARP-1) Alternative names: Poly [ADP-ribose] polymerase 1, 24-kDa form|Poly [ADP-ribose] polymerase 1, 28-kDa form|PARP1|ADPRT|PPOL
• CAS Number: 9007-83-4
• Gene ID: 142
• UniProt: P09874
• Host: Rabbit
• Reactivity: Human, Mouse
• Immunogen: poly(ADP-ribose) polymerase 1
• Target: PARP1
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA, WB
• Field of Research: Metabolism
• Purification: Immunogen affinity purified
• Dilution: WB: 1:500 - 1:2000
• Purity: ≥95% as determined by SDS-PAGE
• Form: Liquid
• Molecular Weight: 115 kDa, 90 kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)