EIF3D antibody

• Background :

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426) .

• Synonyms :

  Eukaryotic translation initiation factor 3 subunit D (eIF3d) |Eukaryotic translation initiation factor 3 subunit 7|eIF-3-zeta|eIF3 p66|EIF3D|EIF3S7

• Gene ID :

  8664

• UniProt :

  O15371

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Eukaryotic translation initiation factor 3, subunit D

• Target :

  EIF3D

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Applications :

  ELISA, WB, IHC

• Field of Research :

  Metabolism

• Purification :

  Protein A+G purification

• Dilution :

  WB: 1:500-1:2000; IHC: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  66 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID :

  2D11

• Tested Applications :

  ELISA, WB, IHC

• Immunogen Sequence :

  320-531aa