5-Ethynyluridine

• Description :

  5-Ethynyluridine (5-EU) is a potent cell-permeable nucleoside can be used to label newly synthesized RNA. 5-Ethynyluridine can be used for isolation and sequencing of nascent RNA from neuronal populations in vivo. 5-Ethynyluridine can be used to identify changes in transcription in vivo in nervous system disease models[1][2]. 5-Ethynyluridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

• Product Name Alternative :

  5-EU

• UNSPSC :

  12352005

• Hazard Statement :

  H302, H315, H319, H335

• Target :

  Biochemical Assay Reagents

• Type :

  Reference compound

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/5-ethynyluridine.html

• Concentration :

  10mM

• Purity :

  99.15

• Solubility :

  DMSO : 100 mg/mL (ultrasonic) |H2O : 25 mg/mL (ultrasonic)

• Smiles :

  OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(C(C#C)=C2)=O)=O)O1

• Molecular Formula :

  C11H12N2O6

• Molecular Weight :

  268.22

• Precautions :

  H302, H315, H319, H335

• References & Citations :

  [1]Van't Sant LJ, et al. In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol Commun. 2021 May 21;9 (1) :94.|[2]Jao CY, et al. Exploring RNA transcription and turnover in vivo by using click chemistry. Proc Natl Acad Sci U S A. 2008 Oct 14;105 (41) :15779-84.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  -20°C, 3 years; 4°C, 2 years (Powder)

• Scientific Category :

  Reference compound1

• Clinical Information :

  No Development Reported

• CAS Number :

  [69075-42-9]