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RACGAP1 Antibody

CAT: 0013-GTR18124198Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18124198Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RACGAP1 Antibody
Product Name Alternative
RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4
UniProt
Q9H0H5
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-GRQGNFFASPMLK
Target
RACGAP1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunohistochemistry:In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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