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Fluo-4 AM

CAT: 0804-HY-101896-02Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0804-HY-101896-02Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm) [1].
CAS Number
273221-67-3
UNSPSC
12171500
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Fluo-4_AM.html
Purity
99.0
Solubility
DMSO : 10 mg/mL (ultrasonic)
Smiles
O=C(OCOC(C)=O)CN(C1=CC=C(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OCOC(C)=O)=C4)=O)C=C1OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
Molecular Formula
C51H50F2N2O23
Molecular Weight
1096.94
References & Citations
[1]Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca (2+) -indicator dyes. Cell Calcium. 2000 Feb;27 (2) :97-106.|[2]Fluo-4.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Fluo-4 AM

Fluo-4 AM is a cell-permeable calcium indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium binding.

CAS Number273221-67-3
PubChem CID4060965
IUPAC Nameacetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-N-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate
Molecular FormulaC51H50F2N2O23
Molecular Weight1096.9
XLogP5.5
Topological Polar Surface Area297
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count27
Rotatable Bond Count36
Heavy Atom Count78
Formal Charge0
Complexity2220
SMILES
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChI
InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
InChIKey
QOMNQGZXFYNBNG-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Fluo-4 AM
CAS: 273221-67-3
CID: 4060965
Formula: C51H50F2N2O23
MW: 1096.9
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight1096.9
XLogP35.5
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count27
Rotatable Bond Count36
Exact Mass1096.27724217
Monoisotopic Mass1096.27724217
Topological Polar Surface Area297 Ų
Heavy Atom Count78
Formal Charge0
Complexity2220
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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