NIPA (Phospho-Ser354) Colorimetric Cell-Based ELISA Kit

• Description:

  The NIPA (Phospho-Ser354) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NIPA (Phospho-Ser354) protein expression profile in cells. The kit can be used for measuring the relative amounts of NIPA (Phospho-Ser354) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NIPA (Phospho-Ser354) .

• Synonyms:

  Nuclear interacting partner of ALK; Nuclear-interacting partner of ALK; ZC3HC1

• Gene Name:

  ZC3HC1

• UniProt:

  Q86WB0

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Essential component of a SCF-type E3 ligase complex, SCF (NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1) . Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF (NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.

• Molecular Weight:

  55262 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Nuclear-interacting partner of ALK

• Subcellular Location:

  Nucleus.