HSP27 (Phospho-Ser82) Colorimetric Cell-Based ELISA Kit

• Description:

  The HSP27 (Phospho-Ser82) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP27 (Phospho-Ser82) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSP27 (Phospho-Ser82) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP27 (Phospho-Ser82) .

• Synonyms:

  Heat shock protein beta-1; HspB1;28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28

• Gene Name:

  HSPB1

• UniProt:

  P04792

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state (PubMed:10383393, PubMed:20178975) . Plays a role in stress resistance and actin organization (PubMed:19166925) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742) .

• Molecular Weight:

  22783 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Heat shock protein beta-1

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub- nuclear structures known as SC35 speckles or nuclear splicing speckles.