SMRC2 Colorimetric Cell-Based ELISA

• Description:

  The SMRC2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SMRC2 protein expression profile in cells. The kit can be used for measuring the relative amounts of SMRC2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SMRC2.

• Synonyms:

  SWI/SNF complex subunit SMARCC2; BRG1-associated factor 170; BAF170; SWI/SNF complex 170 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2; SMARCC2; BAF170

• Gene Name:

  SMARCC2

• UniProt:

  Q8TAQ2

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Ubiquitously expressed.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:11018012) . Can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed:10078207) . May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells (PubMed:12192000) . Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron- specific chromatin remodeling complex (nBAF complex) . During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF) . The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity) . Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (By similarity) .

• Molecular Weight:

  132879 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  SWI/SNF complex subunit SMARCC2

• Subcellular Location:

  Nucleus.