Smad1/5/9 Colorimetric Cell-Based ELISA

• Description:

  The Smad1/5/9 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad1/5/9 protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad1/5/9 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad1/5/9.

• Synonyms:

  Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; BSP1, MADH1, MADR1

• Gene Name:

  SMAD1

• UniProt:

  Q15797

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Ubiquitous. Highest expression seen in the heart and skeletal muscle.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) . SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression.

• Molecular Weight:

  52260 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Mothers against decapentaplegic homolog 1

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271) . Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271) . Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) .