PGP9.5 Colorimetric Cell-Based ELISA Kit

• Description:

  The PGP9.5 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PGP9.5 protein expression profile in cells. The kit can be used for measuring the relative amounts of PGP9.5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PGP9.5.

• Synonyms:

  Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1;3.4.19.12;6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1

• Gene Name:

  UCHL1

• UniProt:

  P09936

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Found in neuronal cell bodies and processes throughout the neocortex (at protein level) . Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

• Molecular Weight:

  24824 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Ubiquitin carboxyl-terminal hydrolase isozyme L1

• Subcellular Location:

  Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. About 30% of total UCHL1 is associated with membranes in brain.