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HINT1 Colorimetric Cell-Based ELISA

CAT: 0519-EKC1732Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1732Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The HINT1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HINT1 protein expression profile in cells. The kit can be used for measuring the relative amounts of HINT1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HINT1.
Synonyms
Histidine triad nucleotide-binding protein 1;3.-.-.-; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1; HINT1; HINT, PKCI1, PRKCNH1
Gene Name
HINT1
UniProt
P49773
Reactivity
Human, Mouse, Rat
Tissue Specificity
Widely expressed.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate) . Hydrolyzes lysyl-AMP (AMP-N-epsilon- (N-alpha-acetyl lysine methyl ester) ) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon- (N-alpha-acetyl lysine methyl ester) ) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.
Molecular Weight
13802 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Histidine triad nucleotide-binding protein 1
Subcellular Location
Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.

UniProtKB · P49773

Adenosine 5'-monophosphoramidase HINT1

HINT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49773
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HINT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.9.1.-
Processing
—
Secondary accessions
Q9H5W8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

ApoptosisTranscriptionTranscription regulationUbl conjugation pathway

Cellular component

CytoplasmNucleus

Disease

Disease variantNeuropathy

Molecular function

HydrolaseProteaseThiol protease

Ligand

Nucleotide-binding

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