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AAK1 Colorimetric Cell-Based ELISA

CAT: 0519-EKC1842Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1842Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The AAK1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AAK1 protein expression profile in cells. The kit can be used for measuring the relative amounts of AAK1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AAK1.
Synonyms
AP2-associated protein kinase 1;2.7.11.1; Adaptor-associated kinase 1; AAK1; KIAA1048
Gene Name
AAK1
UniProt
Q2M2I8
Reactivity
Human, Mouse, Rat
Tissue Specificity
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Molecular Weight
103885 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
AP2-associated protein kinase 1
Subcellular Location
Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity) .

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