DNA Polymerase alpha Colorimetric Cell-Based ELISA

• Description:

  The DNA Polymerase alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DNA Polymerase alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of DNA Polymerase alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DNA Polymerase alpha.

• Synonyms:

  DNA polymerase alpha catalytic subunit;2.7.7.7; DNA polymerase alpha catalytic subunit p180; POLA1; POLA

• Gene Name:

  POLA1

• UniProt:

  P09884

• Reactivity:

  Human

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227) .

• Molecular Weight:

  165913 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  DNA polymerase alpha catalytic subunit

• Subcellular Location:

  Nucleus. Cytoplasm, cytosol. In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern.